Composite

Part:BBa_K3990013:Design

Designed by: Li Wenxi   Group: iGEM21_SMS_Shenzhen   (2021-10-01)


T7+Laccase CotA [E34G;T87A;L182R] Expressing Construct


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 915
    Illegal AgeI site found at 1222
    Illegal AgeI site found at 1378
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We will express the enzyme LaccaseCotA in BL21(DE3) E.coli to decompose the ethylene vinyl acetate copolymer(EVA).


Source

From Bacillus sp.

References